REPROCELL Resources Online
Alvetex Protocols
Alvetex Scaffold
Basics
Alvetex Scaffold Quick Start Protocol
Media Volumes Guide for Different Feeding Options in Alvetex Scaffold in Well Insert formats
Alvetex Scaffold 96-well Plate: Quick Start Protocol
Alvetex Scaffold 96-well plate: Instructions for Use and Example Applications
Alvetex Scaffold 384-well Plate: Quick Start Protocol
Alvetex Scaffold 384-well Plate: Instructions for Use and Example Application
Coating
Poly-D or L-lysine Coating of Alvetex Scaffold
Collagen I Coating of Alvetex Scaffold
Collagen I Thin Gel Coating of Alvetex Scaffold
Matrigel® Coating of Alvetex Scaffold
Fibronectin Coating of Alvetex Scaffold
Cell-Specific
Guidance notes and protocols for the culture of Primary Rat Hepatocytes in Alvetex Scaffold in well insert and well plate formats
Protocol for the culture of primary rat hepatocytes in Alvetex Scaffold 96-well plate format
Culture of the Canine Kidney Epithelial MDCK Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats
Example Protocol for the Culture of the MCF-10A Cell Line on Alvetex Scaffold (15mm disc in 12-well insert format, AVP005 and 96-well plate format, AVP009)
Example Protocol for the Culture of the CaCo2 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats With or Without a Collagen I Gel Layer
Example Protocol for the Co-Culture of the Caco-2 Cell Line with the CCD-18co Cell Line on Alvetex Scaffold in Well Insert Formats
Example Protocol for the Culture of the Breast Carcinoma MDA-MB-231 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats
Example Protocol for the Culture of the Human Colon Fibroblastic CCD-18co Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats
Example Protocol for the Culture of the Lung Carcinoma A549 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats
Example Protocol for the Culture of the Breast Invasive Ductal Carcinoma BT-474 Cell Line on Alvetex Scaffold
Example Protocol for the Culture of the PC-3 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats
Example Protocol for the Culture of the LN-229 Cell Line on Alvetex Scaffold in 96-well Plate Format
Example protocol for the Culture of the 3T3 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats
Example protocol for the culture of the TERA2.cl.SP12 cell line on Alvetex Scaffold
Example protocol for the culture of the HepG2 cell line on Alvetex Scaffold in Well Plate and Well Insert Formats
Example Protocols for the Culture of the CHO-K1 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats
Example Preparation of HaCaT Epidermal Equivalent Using Alvetex Scaffold
Development of Full Thickness Human Skin Model Using Alvetex Scaffold Technology
Example Protocol for the Culture of the SW480 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats
Example Protocol for the Culture of the SW620 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats
Example Protocols for the Co-Culture of either the SW620 or the SW480 Cell Line with the 3T3 Cell Line on Alvetex Scaffold in Well Insert Formats
Example Protocol for the Culture of the MG63 Cell Line on Alvetex Scaffold in Well Insert Formats
Example Protocol for the Culture of Primary Rat MSCs on Alvetex Scaffold in Well Insert Formats
Example Protocols for the Culture of the MCF-7 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats
Transfection
3D Transfection System (Mirus)
Cell Visualization
Simple Visualisation of Cells on Alvetex Scaffold Using Light Microscopy (Neutral Red-Methyl Blue Dye)
Live Cell Imaging of 3D Cultures Grown on Alvetex Scaffold Using Confocal Microscopy
Wheat Germ Agglutinin Staining of 3D Cultures Grown on Alvetex Scaffold and Alvetex Strata
Metabolic Assays
AlamarBlue® Cell Viability Assay of upcyte® hepatocyte Cultures Grown on Alvetex Scaffold in 3D
Determination of Cell Number and Growth Characteristics within Alvetex Scaffold using the PicoGreen® Assay
XTT Cell Viability Assay for Alvetex Scaffold 96-Well Plate Format
MTS Cell Viability Assay of upcyte® Hepatocyte Cultures Grown on Alvetex Scaffold in 3D
MTT cell viability assay of cultures grown on Alvetex Scaffold in 3D
Genomics and Proteomics
Total Protein Extraction from Cells Cultured in Alvetex Scaffold in 3D
Protein Extraction from Cells Cultured in Alvetex Scaffold 96-Well Plate Format
Extraction of RNA from Cells Cultured in Alvetex Scaffold in 3D
RNA Extraction from Cells Cultured in Alvetex Scaffold 96-Well Plate Format
Histology
Histology Series Part 1: Choosing the Right Fixative to Preserve 3D Cell Cultures
Histology Series Part 2: Processing Alvetex Scaffold 3D Cultures for PFA Fixation and Paraffin Wax Embedding for Immunofluorescence
Histology Series Part 3: Processing Alvetex Scaffold 3D Cultures for Bouin’s Fixation and Paraffin Wax Embedding for Haematoxylin and Eosin Staining
Histology Series Part 4: Processing Alvetex Scaffold 3D Cultures for Resin Embedding and Toluidine Blue Staining for Light Microscopy
Histology Series Part 5: Processing Alvetex Scaffold 3D Cultures for Scanning Electron Microscopy (SEM)
Advanced Microscopy
(See also Histology Series Part 5, above.)
Processing Alvetex Scaffold 3D Cultures for Cryosectioning
Immunofluorescence Confocal Microscopy of 3D Cultures Grown on Alvetex Scaffold
Cell Retrieval
Retrieval of Cells from Alvetex Scaffold
Protocol for the removal of Alvetex discs from 6-well or 12-well inserts by unclipping the well insert base
Alvetex Strata
Alvetex Strata Quick Start Protocol
Retrieval of Cells from Alvetex Strata
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