Alvetex Protocols

Alvetex Scaffold

Basics

Alvetex Scaffold Quick Start Protocol

Media Volumes Guide for Different Feeding Options in Alvetex Scaffold in Well Insert formats

Alvetex Scaffold 96-well Plate: Quick Start Protocol

Alvetex Scaffold 96-well plate: Instructions for Use and Example Applications

Alvetex Scaffold 384-well Plate: Quick Start Protocol

Alvetex Scaffold 384-well Plate: Instructions for Use and Example Application

Coating

Poly-D or L-lysine Coating of Alvetex Scaffold

Collagen I Coating of Alvetex Scaffold

Collagen I Thin Gel Coating of Alvetex Scaffold

Matrigel® Coating of Alvetex Scaffold

Fibronectin Coating of Alvetex Scaffold

Cell-Specific

Guidance notes and protocols for the culture of Primary Rat Hepatocytes in Alvetex Scaffold in well insert and well plate formats

Protocol for the culture of primary rat hepatocytes in Alvetex Scaffold 96-well plate format

Culture of the Canine Kidney Epithelial MDCK Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats

Example Protocol for the Culture of the MCF-10A Cell Line on Alvetex Scaffold (15mm disc in 12-well insert format, AVP005 and 96-well plate format, AVP009)

Example Protocol for the Culture of the CaCo2 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats With or Without a Collagen I Gel Layer

Example Protocol for the Co-Culture of the Caco-2 Cell Line with the CCD-18co Cell Line on Alvetex Scaffold in Well Insert Formats

Example Protocol for the Culture of the Breast Carcinoma MDA-MB-231 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats

Example Protocol for the Culture of the Human Colon Fibroblastic CCD-18co Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats

Example Protocol for the Culture of the Lung Carcinoma A549 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats

Example Protocol for the Culture of the Breast Invasive Ductal Carcinoma BT-474 Cell Line on Alvetex Scaffold

Example Protocol for the Culture of the PC-3 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats

Example Protocol for the Culture of the LN-229 Cell Line on Alvetex Scaffold in 96-well Plate Format

Example protocol for the Culture of the 3T3 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats

Example protocol for the culture of the TERA2.cl.SP12 cell line on Alvetex Scaffold

Example protocol for the culture of the HepG2 cell line on Alvetex Scaffold in Well Plate and Well Insert Formats

Example Protocols for the Culture of the CHO-K1 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats

Example Preparation of HaCaT Epidermal Equivalent Using Alvetex Scaffold

Development of Full Thickness Human Skin Model Using Alvetex Scaffold Technology

Example Protocol for the Culture of the SW480 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats

Example Protocol for the Culture of the SW620 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats

Example Protocols for the Co-Culture of either the SW620 or the SW480 Cell Line with the 3T3 Cell Line on Alvetex Scaffold in Well Insert Formats

Example Protocol for the Culture of the MG63 Cell Line on Alvetex Scaffold in Well Insert Formats

Example Protocol for the Culture of Primary Rat MSCs on Alvetex Scaffold in Well Insert Formats

Example Protocols for the Culture of the MCF-7 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats

Transfection

3D Transfection System (Mirus)

Cell Visualization

Simple Visualisation of Cells on Alvetex Scaffold Using Light Microscopy (Neutral Red-Methyl Blue Dye)

Live Cell Imaging of 3D Cultures Grown on Alvetex Scaffold Using Confocal Microscopy

Wheat Germ Agglutinin Staining of 3D Cultures Grown on Alvetex Scaffold and Alvetex Strata

Metabolic Assays

AlamarBlue® Cell Viability Assay of upcyte® hepatocyte Cultures Grown on Alvetex Scaffold in 3D

Determination of Cell Number and Growth Characteristics within Alvetex Scaffold using the PicoGreen® Assay

XTT Cell Viability Assay for Alvetex Scaffold 96-Well Plate Format

MTS Cell Viability Assay of upcyte® Hepatocyte Cultures Grown on Alvetex Scaffold in 3D

MTT cell viability assay of cultures grown on Alvetex Scaffold in 3D

Genomics and Proteomics

Total Protein Extraction from Cells Cultured in Alvetex Scaffold in 3D

Protein Extraction from Cells Cultured in Alvetex Scaffold 96-Well Plate Format

Extraction of RNA from Cells Cultured in Alvetex Scaffold in 3D

RNA Extraction from Cells Cultured in Alvetex Scaffold 96-Well Plate Format

Histology

Histology Series Part 1: Choosing the Right Fixative to Preserve 3D Cell Cultures

Histology Series Part 2: Processing Alvetex Scaffold 3D Cultures for PFA Fixation and Paraffin Wax Embedding for Immunofluorescence

Histology Series Part 3: Processing Alvetex Scaffold 3D Cultures for Bouin’s Fixation and Paraffin Wax Embedding for Haematoxylin and Eosin Staining

Histology Series Part 4: Processing Alvetex Scaffold 3D Cultures for Resin Embedding and Toluidine Blue Staining for Light Microscopy

Histology Series Part 5: Processing Alvetex Scaffold 3D Cultures for Scanning Electron Microscopy (SEM)

Advanced Microscopy

(See also Histology Series Part 5, above.)

Processing Alvetex Scaffold 3D Cultures for Cryosectioning

Immunofluorescence Confocal Microscopy of 3D Cultures Grown on Alvetex Scaffold

Cell Retrieval

Retrieval of Cells from Alvetex Scaffold

Protocol for the removal of Alvetex discs from 6-well or 12-well inserts by unclipping the well insert base

Alvetex Strata

Alvetex Strata Quick Start Protocol

Retrieval of Cells from Alvetex Strata